Table 3.
Substrate | Coenzyme | Yield of ketone reduction (%) |
---|---|---|
TFA | NADPH | 16 (1.00)* |
NADH | 3.5 (0.22)* | |
PF | NADPH | 12 (1.00)* |
NADH | 0.8 (0.07)* |
Substrate: 2 mg, 0.1 M potassium phosphate buffer (pH7): 2 mL,
Acetone-dried powder of Synechococcus: 10 mg, NADH or NADPH: 5 mg, 25°C, 44 h
*Values in parentheses are the relative to the yields in the presence of NADPH.