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. 2011 Sep 1;1:24. doi: 10.1186/2191-0855-1-24

Table 3.

Coenzyme (NADH/NADPH) dependency on ketone reduction using cell-free extracts of Synechococcus

Substrate Coenzyme Yield of ketone reduction (%)
TFA NADPH 16 (1.00)*
NADH 3.5 (0.22)*
PF NADPH 12 (1.00)*
NADH 0.8 (0.07)*

Substrate: 2 mg, 0.1 M potassium phosphate buffer (pH7): 2 mL,

Acetone-dried powder of Synechococcus: 10 mg, NADH or NADPH: 5 mg, 25°C, 44 h

*Values in parentheses are the relative to the yields in the presence of NADPH.