Figure 2.
Confirmation of PD-1 expression in adult RGCs. (A) Double staining of PD-1 with NeuN on vertical retina sections. GCL is shown, with top to bottom panels representing NeuN (red), PD-1 (green), merge (yellow), and nuclear DAPI (blue) staining. Scale bar, 50μm. (B) Quantification of NeuN positive population in vivo. Percentage of PD-1 positive (99±0.2%) and PD-1 negative (1.2±0.2%) cells among NeuN positive RGCs is shown. These findings are representative of three experiments.