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Inactivation kinetics of extractable NR from N. plumbaginifolia leaves in response to a light-dark transition. Plants grown in the greenhouse with light (L) were put in a dark room (t = 0) and kept in darkness for various times. Leaves were harvested from two different plants and NR was extracted in a magnesium-containing buffer. NR activity was measured in the crude extracts with 15 mm EDTA (▪) or without EDTA (○).
