Figure 8.

NR total activity in leaves of wild-type and transgenic N. plumbaginifolia plants during prolonged darkness. Leaves were harvested as described in the legend to Figure 7. NR activity was then assayed in the crude extracts for 10 min in the presence of 15 mm EDTA. The NR activities shown correspond to the NR activities measured with EDTA in the experiment described in Figure 7. NR activity is given as a percentage of the NR activity measured in the light (L, 100%). Values for specific NR activities in the light (in nanomoles of nitrite produced per minute per milligram of protein) were 24 for wild type, 23 for C1, 11 for del8, 15 for A1, 14 for A2, 12 for S2, 13 for S4, and 11 for S9.