Figure 3.

The effect of Ca²⁺ on the motile activity of 170-kD myosin. A, Effect of Ca²⁺ on the percentage of translocated RP-labeled F-actin (•) and velocity of translocation (▪). B, Reversibility of the Ca²⁺ inhibition of 170-kD myosin. The percentage of translocated RP-labeled F-actin was measured using an assay medium containing Ca²⁺ of various concentrations (white bars). After the flow chamber was perfused with an assay medium lacking CaCl₂, the percentage of translocated RP-labeled F-actin was again measured (black bars). The results presented are typical of three separate experiments.