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Electrophoretic mobility shift in SDS-PAGE by Ca2+. Immunoblotting of 170-kD myosin (A and B) and spinach CaM (C and D) was carried out using antiserum against CaM. Each sample was treated with SDS-PAGE sample buffer supplemented with either 2 mm CaCl2 (A and C) or 1 mm EGTA (B and D) and subjected to SDS-PAGE on a 15% polyacrylamide gel. Mrs (×10−3) of standard proteins are indicated on the left.
