Figure 8.
Purity of CaM isolated from lily pollen (A) and the effect of CaM on the motile activity of 170-kD myosin inactivated by 10−4 m Ca2+ (B). A, Coomassie brilliant blue staining of 15% polyacrylamide gel. B, Effect of CaM on the percentage of translocated RP-labeled F-actin. White bars, The coverslip coated with 170-kD myosin was rinsed by a solution containing 10−4 m Ca2+ and 2 μm CaM and then used for a motility assay in an assay medium containing Ca2+ at concentrations of 10−6 m (pCa 6), 10−7 m (pCa 7), or EGTA (E). Black bars, CaM (2 μm) was supplied to both the rinsing solution and the assay medium. Mrs (×10−3) of standard proteins are indicated on the left in A.