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Typical appearances for AIF immunohistochemistry (brown regions staining positive, blue negative) (A–D all GBM). (A) Tumor with absent AIF staining. (B) Low level staining (+). (C) Moderate staining (++). (D) High staining (+++). (E) Nonneoplastic brain from adjacent to tumor demonstrating moderate levels of AIF staining in neurons (*). (F) Nonneoplastic brain demonstrating AIF immunopositivity in glia (*). All views x400 magnification.
