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. 2011 May 31;19(11):1950–1960. doi: 10.1038/mt.2011.107

Figure 5.

Figure 5

Transplacental viral trafficking and maternal outcomes. Maternal viraemia available from 10 mothers (a, AAV5 n = 7, AAV8 n = 3) followed IUGT with both vectors, detectable 5 minutes after injection and resolving 72 hours later (qPCR sensitivity down to 1.5 copies). Median VCN of paired tissue biopsies taken at birth were compared in all 11 mother-infant pairs (b, AAV5 n = 7, AAV8 n = 4 with the exception of 1263/8-001). Median VCN in maternal tissues was between one and four-log fold lower than median VCN in corresponding infant tissues. (c,d) Mothers developed an immune response toward viral capsids; (d) the response was more sustained after AAV5 (c) compared to AAV8. Transgene expression was liver-specific but hFIX was undetectable in maternal plasma for both (e) AAV8 and (f) AAV5. Gel images were recorded as described. (g) Cumulus cell-free oocytes were found to be free of vector sequences whereas cumulus cells themselves contained minute quantities of proviral DNA. AAV, adeno-associated viral vector; hFIX, human Factor IX; IUGT, intrauterine gene transfer; VCN, vector copy number.