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. 2011 Aug 9;19(11):1981–1989. doi: 10.1038/mt.2011.160

Figure 5.

Figure 5

In vivo gene transfer to airways of C57 mice intranasally challenged with Pseudomonas aeruginosa lipopolysaccharide (LPS). Periodic acid Schiff (PAS) staining of paraffin sections of lung tissues from mice treated/challenged twice either with (a) isotonic saline or (b) 2 mg/ml LPS in the interval of 2 days. On day 4, PAS-positive mucus cells (magenta color; indicated with triangles) were detected in the airways of LPS-challenged mice whereas no PAS-positive cells were detected in the airways of saline-treated mice. (c) Total mucin contents in bronchioalveolar lavage fluid (BALF) collected from mice treated/challenged with isotonic saline or LPS. (d) In vivo gene transfer to airways of mice treated/challenged twice either with saline or LPS. On day 4, mice were treated with adjuvants, either saline (n = 7) or NAC solution (n = 10) with varying concentrations (0.1 and 0.5 mol/l) 30 minutes prior to the administration of CK30PEG10k/DNA NPs carrying pd1GL3-RL (luciferase). Subsequently, CK30PEG10k/DNA NPs (DNA dose of 50 µg/mouse) were intranasally instilled to each mouse and the luciferase activity was measured 24 hours after the administration. The differences are statistically significant between the conditions indicated with asterisks (*) (*P < 0.05, **P < 0.01, ***P < 0.001). Mean ± SEM.