Figure 3.
ELISPOTs for interferon-γ (IFN-γ) and interleukin-2 (IL-2) responses and neutralizing antibodies to adeno-associated virus serotype 8 (AAV8) in nonhuman primates (NHPs). Freshly isolated peripheral blood mononuclear cells (PBMCs) from naive rhesus macaques were test by an ELISpot assay for IFN-γ and IL-2 secretion upon stimulation with four different peptide pools containing overlapping peptides of the AAV8 capsid. The graphs showed responses of 21 animals. Responses to medium (negative control) were subtracted from responses to each pool. (a,b) Our cut-off for positive responses was 55 spots/106 PBMCs. Error bar showed the SD of two different wells. (c) Plasmas from these rhesus macaques were also collected and titers of neutralizing antibodies to AAV8 were tested.