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. Author manuscript; available in PMC: 2011 Nov 22.
Published in final edited form as: Biochemistry. 2006 Jul 25;45(29):8894–8902. doi: 10.1021/bi060534y

Figure 5.

Figure 5

Absorption spectra of DMXBA 18 and 2-MeO and 4-OHBA 21 in the presence of different solvents and bound to the AChBP. (A) and (B) DMXBA and 2-MeO and 4-OHBA in the presence of methanol (εr = 33.0), acetonitrile (εr = 37.5), H2O (εr = 80.0), and a 0.1 M phosphate buffer (PB) at pH 7.0 (εr ~ 80.0). (C) and (D) DMXBA and 2-MeO and 4-OHBA absorbance of ligands in a 0.1 M phosphate buffer at pH 7.0 (−), bound to AChBP. (E) and (F) Difference spectra reflecting DMXBA (E) and 2-MeO, 4-OHBA (F) in mixed (sample cuvette) and unmixed (reference cuvette) tandem compartments. Substituted BAs (20 µM) were used in the presence of 1.2 M excess of AChBP sites in 0.42 path length cuvettes. The difference spectra were measured comparing mixed and unmixed 20 µM BA and 24 µM AChBP in the same tandem cuvettes. The absorption values in the difference spectra are corrected for a 1 cm path length.