Figure 3.

Detection of Mt4-like genes in Arabidopsis, tomato, and soybean. A, Northern blot of total RNA isolated from plants receiving either low-Pi (−Pi) or high-Pi (+Pi) fertilizer. The blot was probed with the conserved sequence (Oligo), the Mt4 cDNA, the At4 cDNA, or a ³²P-labeled pSR1-2B3 (rRNA). B, Expression of At4 in Arabidopsis roots. RT-PCR products from RNA extracted from leaves and roots of Pi-deprived Arabidopsis plants (wild type and pho1 mutant) using At4-specific primers. The blot was hybridized with ³²P-labeled At4 cDNA.