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. 1999 Jan;119(1):241–248. doi: 10.1104/pp.119.1.241

Figure 7.

Figure 7

Southern blots of RT-PCR products derived from wild-type Arabidopsis and the Pi-accumulation mutant pho1. Total RNA was extracted from plants grown with (+Pi) or without (−Pi) phosphate fertilization, reverse transcribed, standardized, amplified by PCR using a primer pair based on the At4 sequence, blotted, and probed with either a 32P-labeled At4 cDNA (At4) or an end-labeled oligonucleotide identical to the conserved sequence (Oligo) for two independent experiments (A and B).