Southern blots of RT-PCR products derived from
wild-type Arabidopsis and the Pi-accumulation mutant
pho1. Total RNA was extracted from plants grown with
(+Pi) or without (−Pi) phosphate fertilization, reverse transcribed,
standardized, amplified by PCR using a primer pair based on the At4
sequence, blotted, and probed with either a 32P-labeled At4
cDNA (At4) or an end-labeled oligonucleotide identical to the conserved
sequence (Oligo) for two independent experiments (A and B).