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. 2011 Nov 1;10:133. doi: 10.1186/1476-4598-10-133

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Copyright ©2011 Xu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ad.SPDD-HCCS1 can induce apoptosis of hepatocellular carcinoma cells both in vitro and in vivo. (A) Ad.SPDD-HCCS1 and Ad.SPDD-HCCS1HA induce more nuclear condensation than Ad.SPDD. Hoechst33258 staining was performed on Huh-7 cells 48 hours after infection with viruses at an MOI of 10. Scale bar = 20 μm. (B) Annexin V-FITC/PI staining FACS assay. The percentages of the cells in the second and fourth quadrants are shown. (C) Detection of apoptosis in tumor sections by TUNEL staining. Scale bar = 100 μm.