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. 2011 Nov 22;6(11):e27601. doi: 10.1371/journal.pone.0027601

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Maguire et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of kisspeptin receptor mRNA in samples (n = 3) of human myometrium (Lanes 1–3), used as a control to confirm the specificity of PCR primers as this is a tissue in which the receptor protein has been previously identified. The integrity of the samples was confirmed by detection of the β-actin product of the expected size, 353 bp, indicating integrity of cDNA and absence of gDNA. PCR product size was determined using a 100 bp DNA ladder (Lane 4) (B) Expression of kisspeptin receptor mRNA (expected size 198 bp) in cDNA from human isolated cardiomyocyte samples (n = 3, lanes 3–5). β-Actin control (Lane 2) acted as a positive control and the absence of cDNA (Lane 1) served as a negative control with a 100 bp DNA ladder (Lane 6).