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. 2011 Nov 22;6(11):e27601. doi: 10.1371/journal.pone.0027601

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Maguire et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Western blot analysis of rabbit anti-kisspeptin receptor (230–244, rat/mouse) serum on membrane preparations of (A) rat (n = 3) and (B) mouse (n = 3) heart revealed a single band of the expected size, 43 kDa or 75 kDa respectively (lanes 1–3). Pre-absorption of the primary antibody with the immunizing peptide resulted in attenuation of the bands (lanes 4–6). (C) Human left ventricle (n = 4) was probed with rabbit anti-kisspeptin receptor (375–398, human) (lanes 1–4) producing a band at 42 kDa, the expected size, which was attenuated when the primary antibody was pre-absorbed with the immunizing peptide (lanes 5–8). Protein marker positions are shown alongside the blots.