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. 2011 Nov 22;6(11):e27729. doi: 10.1371/journal.pone.0027729

Figure 7. Loss of homeostasis and function of MDSC in splenocytes from TB mice.

Figure 7

(A) Western blot analysis of phospho-AKT-1 (Ser473) and BCL-2 protein expression in Gr-1+ enriched MDSC splenocytes from TB and control mice. To control for equal protein loading the blot was re-probed with an antibody specific for β-actin. (B) TB Gr1+ MDSC significantly suppress antigen-specific T cell responses (OT-I specific (CD8+) T cells primed with OVA-peptide pulsed DC (DC-OVA)) compared to control Gr1+ MDSC in vitro. Controls for the assay included T cells incubated alone in complete medium (CM) or DC-OVA. Control (n = 3) compared to TB (n = 3) mice. **p<0.01; ***p<0.001 (by two-tailed Student's t test).