Table I.
Isolation of cello-oligosaccharides from apoplastic solution
| Ethanol Fractionation | Total Sugara | Cello-Oligosaccharidesb | Isolated
Cello-Oligosaccharides
|
||
|---|---|---|---|---|---|
| Cellobiose | Cellotriose | Cellotetraose | |||
| % | μg | ||||
| 0 | 5,100 | 0 | 0 | 0 | 0 |
| 2.5 | 186,000 | 0 | 0 | 0 | 0 |
| 5 | 2,200 | 362 | 113 | — | — |
| 10 | 480 | 284 | 99 | 19 | — |
| 15 | 780 | 213 | — | 47 | 28 |
| 20 | 2,500 | 242 | — | — | 32 |
a
Values were determined by the phenol/sulfuric acid method (Dubois et al., 1956).
b
Values were determined by using cellobiose dehydrogenase (Samejima and Eriksson, 1992).