Table II.
Substrate specificity of cellobiose dehydrogenase
| Sugar (2 μm) | Relative Activity |
|---|---|
| % | |
| Cellobiose | 100.0 |
| Cellotriose | 88.1 |
| Cellotetraose | 79.0 |
| Cellopentaose | 72.4 |
| Cellohexaose | 72.0 |
| Laminaribiose | 0 |
| Laminaritriose | 0 |
| Maltose | 0.1 |
| Suc | 0 |
| Raffinose | 0.1 |
| Glc | 0.7 |
| Fru | 0 |
| Isoprimeverose | 0 |
| XXG | 0 |
| XXXG | 0 |
| XXXGXXXG | 0 |
The reaction mixtures contained 90 milliunits of cellobiose dehydrogenase, 5 nm Cyt c, 2 nmol of substrate, and 0.1 m sodium acetate (pH 4.2) in a total volume of 100 μL. After incubation for 5 min, the A550 was determined.