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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Bone Miner Res. 2011 Dec;26(12):2948–2958. doi: 10.1002/jbmr.511

Figure 3. Effect of skeletal unloading on the activation of PDGF and IGF-I receptors.

Figure 3

BMOp cells from loaded and unloaded bones were serum deprived at day 7 in culture, and then treated with IGF-I (A) or PDGF (B). Representative immunoblots illustrate skeletal unloading induced impairment of IGF-I receptor phosphorylation. Relative signal intensities of the ratio of phosphorylated to total IGF-I or PDGF receptors were evaluated. Means ± SD, n = 3. a p < 0.05 vs. Loaded. (C) BMOp cells from loaded and unloaded bones were serum deprived at day 7 in culture, and then exposed to 25pM 125I-IGF and various doses of unlabeled IGF-I or des-IGF-I for 10 minutes. Data are means of triplicates. Error bars are not shown to simplify presentation, but in all cases enclosed an SD of less than 10% of the mean. These values are expressed as a percentage of maximal specific 125I-IGF binding that was comparable for BMOp cells isolated from loaded and unloaded bones.