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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Neurochem Int. 2011 Jul 3;61(5):807–814. doi: 10.1016/j.neuint.2011.06.011

Fig.8.

Fig.8

Effect of the calcium chelator, BAPTA-AM on gp120-induced gene and protein expression: Cultured of human astrocytes (1×106 cells/ml) were pretreated with the calcium inhibitor BAPTA-AM for 30 minutes prior to the treatment with 50ng/ml of gp120 recombinant protein for 48 h. At the end of the treatment periods, cells were harvested, RNA and protein were extracted. RNA extracted from different groups of cells was reverse transcribed and analyzed for the mRNA abundance levels employing qRTPCR using the specific primers against Nrf2, HO-1 and Nqo1 (A). The protein samples were immune-blotted against anti-Nrf2, anti-HO-1 and anti-Nqo1. The figure shows the representative blots of Nrf2, HO-1 and Nqo1 expression (B). Quantification of the protein band intensities normalized against actin (C). Data were obtained from three independent experiments and represented as mean ± SEM. *P<0.05 compared to control; †compared to gp120, P<0.05.