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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Bone Miner Res. 2011 Dec;26(12):2923–2934. doi: 10.1002/jbmr.494

Fig. 2. Proteasome inhibition leads to PTHR accumulation.

Fig. 2

CHO-N10-R3 (A), HK-2 (B) or ROS (C) cells were pre-treated with the proteasome inhibitor MG-132 at 25 μM for 1 hour before addition of either 100 nM PTH(1–84), 1 μM PTH(7–34) or 1 μM PTH(7–84) as indicated for an additional 6 hours at 37°C. Equal amounts of cell lysates were analyzed by immunoblot for HA-PTHR (A and C) or endogenous PTHR (B) and shown as the relative abundance as a percentage of total receptor abundance in untreated cells. Data illustrate 5 (A and B) or 4 (C) independent experiments. *p < 0.05; **p < 0.01 vs. corresponding vehicle condition.