Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Bone Miner Res. 2011 Dec;26(12):2923–2934. doi: 10.1002/jbmr.494

Fig. 4. PTHR ubiquitination.

Fig. 4

ROS cells transfected with HA-PTHR and Myc-ubiquitin were pre-treated with MG-132 (25 μM; 1 hour) before incubation with either 100 nM PTH(1–34) or 1 μM PTH(7–34) for 5–120 minutes at 37°C. Cell lysates were immunoprecipitated using monoclonal anti-HA beads. Typical immunoblot of immunoprecipitates probed with polyclonal ubiquitin antibody (A, C) or Lys48 –specific ubiquitin antibody (D) are shown. Representative images of 3 independent experiments.