Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Bone Miner Res. 2011 Dec;26(12):2923–2934. doi: 10.1002/jbmr.494

Fig. 5. PTHR is ubiquitinated at the plasma membrane.

Fig. 5

ROS cells were transfected with HA-PTHR. After 48 hours, the cells were treated with 100 nM PTH(1–34) or 1 μM PTH(7–34) for 5 minutes. Membrane proteins were isolated and immunoprecipitated using monoclonal anti-HA beads as described in Materials and Methods. Representative immunoblot of immunoprecipitate probed with polyclonal ubiquitin antibody. Values are mean ± SEM from 3 independent experiments.