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. Author manuscript; available in PMC: 2012 Dec 1.

Published in final edited form as: Hepatology. 2011 Dec;54(6):1975–1986. doi: 10.1002/hep.24607

Fig. 3 — Liver microsomes were isolated from 3-month-old (3m) and 8-month-old (8m) wild type (WT) (□) and MAT1A-knockout (MAT1A-KO) () mice by differential centrifugation. (A–D) Lipids were extracted from liver microsomes, and free fatty acid (FFA), unesterified cholesterol (UC), lysophosphatidylcholine (LPC), sphingomyelin (SM), phosphatidylserine (PS), phosphatidylinositol (PI), cardiolipin (Cln), triglyceride (TG), cholesteryl ester (CE), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were separated and quantified as indicated in material and methods. (E) Microsomal phosphatidylethanolamine N-methyltransferase (PEMT) activity was determined by a radiometric assay. Values are means ± SEM of 4–8 animals per group. Statistical differences between MAT1A-KO and WT mice are denoted by *p<0.05 (Student's t test).

Inline graphic — Liver microsomes were isolated from 3-month-old (3m) and 8-month-old (8m) wild type (WT) (□) and MAT1A-knockout (MAT1A-KO) () mice by differential centrifugation. (A–D) Lipids were extracted from liver microsomes, and free fatty acid (FFA), unesterified cholesterol (UC), lysophosphatidylcholine (LPC), sphingomyelin (SM), phosphatidylserine (PS), phosphatidylinositol (PI), cardiolipin (Cln), triglyceride (TG), cholesteryl ester (CE), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were separated and quantified as indicated in material and methods. (E) Microsomal phosphatidylethanolamine N-methyltransferase (PEMT) activity was determined by a radiometric assay. Values are means ± SEM of 4–8 animals per group. Statistical differences between MAT1A-KO and WT mice are denoted by *p<0.05 (Student's t test).