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. Author manuscript; available in PMC: 2012 Dec 1.

Published in final edited form as: Hepatology. 2011 Dec;54(6):1975–1986. doi: 10.1002/hep.24607

Fig. 8 — 3-month-old wild type (WT) (□), MAT1A-knockout (MAT1A-KO) () and SAMe treated MAT1A-KO (KO + SAMe) (■) mice were fasted 2 hours prior to the injection of 1 mg/g poloxamer (P-407) in saline to inhibit VLDL metabolism. Before P-407 injection and 6 hours later blood was collected and VLDL (d<1.02 g/ml) were isolated from serum by ultracentrifugation and characterized for (A) apoB, (B) particle size, (C) triglyceride (TG) and cholesterol (chol) content. (D) Liver microsomes were prepared and microsomal phosphatidylethanolamine N-methyltransferase (PEMT), diacylglycerol acyltransferase (DGAT) and TG lipase activities were determined as described in materials and methods. (E) Serum was isolated and levels of ketone bodies (KB) and free fatty acids (FFA) were measured. Values are means ± SEM of 4–8 animals per group. Statistical differences versus WT mice are denoted by *p<0.05, **p<0.01 and ***p<0.001 and versus MAT1A-KO mice are denoted by ^#p<0.05, ^##p<0.01 and ^###p<0.001 (Student's t test).

Inline graphic — 3-month-old wild type (WT) (□), MAT1A-knockout (MAT1A-KO) () and SAMe treated MAT1A-KO (KO + SAMe) (■) mice were fasted 2 hours prior to the injection of 1 mg/g poloxamer (P-407) in saline to inhibit VLDL metabolism. Before P-407 injection and 6 hours later blood was collected and VLDL (d<1.02 g/ml) were isolated from serum by ultracentrifugation and characterized for (A) apoB, (B) particle size, (C) triglyceride (TG) and cholesterol (chol) content. (D) Liver microsomes were prepared and microsomal phosphatidylethanolamine N-methyltransferase (PEMT), diacylglycerol acyltransferase (DGAT) and TG lipase activities were determined as described in materials and methods. (E) Serum was isolated and levels of ketone bodies (KB) and free fatty acids (FFA) were measured. Values are means ± SEM of 4–8 animals per group. Statistical differences versus WT mice are denoted by *p<0.05, **p<0.01 and ***p<0.001 and versus MAT1A-KO mice are denoted by ^#p<0.05, ^##p<0.01 and ^###p<0.001 (Student's t test).