Table 2.
Oligo and Primer Sequences Used for Reporter Constructs
| Reporter construct | Annealing oligos and primer sequences |
|---|---|
| p6xNone-Fwd (−4284 to −4232 bp) | 5′-GATCCACGTAGAATAAGCTCCTTCATAAAGTCACAGACCAGTCAGGCTGAACAGCTCCA-3′ (GATCC/A: BamH I / Bgl II adapter) |
| p6xNone-Rev | 5′-GATCTGGAGCTGTTCAGCCTGACTGGTCTGTGACTTTATGAAGGAGCTTATTCTACGTG-3′ (GATCT / G: Bgl II / BamH I adapter) |
| p6xTgtggg-Fwd (−4234 to −4182 bp) | 5′-GATCCTCCGAGGAAACACCCAGAATAAAAATAGTTTAATACACACAATTAGG TGTGGG A-3′ (GATCC / A: BamH I / Bgl II adapter, TGTGGG: putative Runx2 binding site) |
| p6xTgtggg-Rev | 5′-GATCTCCCACACCTAATTGTGTGTATTAAACTATTTTTATTCTGGGTGTTTCCTCGGAG-3′ (GATCT / G: Bgl II / BamH I adapter) |
| p6xTgtggc-Fwd (−4184 to −4132 bp) | 5′-GATCCGGG TGTGGC CAGCAAATACTCTGATTCTACAATCTGTTTTGGACAGGGCAGTGA-3′ (GATCC / A: BamH I / Bgl II adapter, TGTGGC: putative Runx2 binding site) |
| p6xTgtggc-Rev | 5′-GATCTCACTGCCCTGTCCAAAACAGATTGTAGAATCAGAGTATTTGCTGGCCACACCCG-3′ (GATCT / G: Bgl II / BamH I adapter) |
| Tg-6x83-Fwd (−4296 to −4214 bp) | 5′-AATGGATCCTCCTGTTTCACGTAG-3′ (GGATCC: BamH I linker) |
| Tg-6x83-Rev | 5′-TTTAGATCTATTCTGGGTGTTTCC-3′ (AGATCT: Bgl II linker) |
| Tg-6x88-Fwd (−4234 to −4147 bp) | 5′-ACAGGATCCGAGGAAACACC-3′ (GGATCC: BamH I linker) |
| Tg-6x88-Rev | 5′-AACAGATCTGTAGAATCAGAG-3′ (AGATCT: Bgl II linker) |
| Tg-12xLP3-Fwd (−4201 to −4163 bp) | 5′-GATCCTACACACAATTAGGTGTGGGTGTGGCCAGCAAATACTCTA-3′ (GATCC / A: BamH I / Bgl II adapter) |
| Tg-12xLP3-Rev | 5′-GATCTAGAGTATTTGCTGGCCACACCCACACCTAATTGTGTGTAGG-3′ (GATCT / G: Bgl II / BamH I adapter) |
Box = putative Runx2 binding sites; GATCC/A = BamH I/Bgl II adapter.