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Covalent multilayer films were functionalized with either solutions containing RGD or the scrambled negative control peptide, RDG. hTCEpi cells seeded onto RDG surfaces (a–g) were unable to attach. hTCEpi cells seeded onto RGD surfaces (h–n) attached to spots fabricated from 3 to 20% RGD/d-glucamine solutions. Cells were allowed to attach for 24 hours and were then stained with SYTO-11 green fluorescent nucleic acid stain.Scale bar equals 1 mm.
