Fig. 2.
Inhibition of quercetin-evoked outward current by a K channel blocker in 253J cells. Voltage steps of 150 ms duration were applied from -120 mV to 100 mV in 20 mV increments every 10 sec. (A) Outward current activated by 50 µM quercetin was inhibited by TEA and recovered after TEA washout. (B) The specific BKCa channel inhibitor, IBX, inhibited ~97% of the quercetin-evoked whole cell outward current and the current was almost irreversible with IBX-free solution including quercetin in bladder cancer cells. (C) Dosedependent effects of quercetin on bladder cancer cells at 60 mV were plotted (n=5 cells per concentration). Quercetin substantially activated the outward current dose dependently. Quercetin-evoked current continually recorded for 150 ms ramp pulse from -100 to 100 mV at a holding potential of -60 mV. The bath solution contained normal Tyrode's (NT) and 0 µM, 1 µM, 10 µM, 30 µM, 50 µM, and 100 µM quercetin. Each point represents the mean ± standard deviation of the mean (SD) (n=5).