Figure 1.

A, Overlapping bacteriophage λ clones provide the full-length Zpu1 cDNA. The 3261-bp nucleotide sequence of the Zpu1 cDNA was determined from overlapping sequences of three λ clones. The Zpu1 cDNA sequence is available as accession no. AF080567. B, Primary sequence alignment of ZPU1 with rice RE. Amino acid residues deduced from the Zpu1 cDNA (Zm) and rice RE cDNA (Os; accession no. D50602) are shown in alignment. Boxed residues are the same in both polypeptides. The asterisk designates the transit peptide cleavage site known in RE.