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The copy number of the zpu1 locus was determined by gel-blot analysis of genomic DNA. DNA from maize inbred W64A was digested with the indicated restriction enzymes (B, BamHI; K, KpnI; N, NotI; P, PstI; S, SstI; X, XbaI; and Xh, XhoI). The gel blot was hybridized at high stringency with probe EE2.3 (left panel), then stripped of probe and hybridized with probe EH.68 (right panel) of the Zpu1 cDNA.
