Table I.
Purification of ZPU1a
| Step | Total Protein | Total Activity | Specific Activity | Purification | Recovery |
|---|---|---|---|---|---|
| mg | unitsb | units/mg | fold | % | |
| Purification A | |||||
| Crude extract | 328 | 6.56 | 0.02 | NDc | ND |
| Ammonium sulfate (40%) | 146 | 1.46 | 0.01 | 1 | 100 |
| Q-Sepharose | 17 | 0.85 | 0.05 | 5 | 58.2 |
| Sephacryl S-200 | 3 | 0.75 | 0.25 | 25 | 51.4 |
| Mono-Q | 0.01 | 0.01 | 1.00 | 100 | 0.68 |
| Purification B | |||||
| Crude extract | 288 | 20.16 | 0.07 | ND | ND |
| Ammonium sulfate (40%) | 104 | 2.09 | 0.02 | 1 | 100 |
| Q-Sepharose | 4 | 0.42 | 0.11 | 5.5 | 20.1 |
| Cyclohexa-amylose | 0.06 | 0.24 | 3.99 | 199.5 | 11.4 |
a
Total and specific activities are apparent values, because contaminating hydrolytic enzymes may cleave the substrates or products of the assay reactions.
b
Units are micromole maltotriose equivalents per minute.
c
ND, Not determined because of potential inaccuracy resulting from contaminating hydrolases.