Fig. 3.

Expression patterns of ECM-related factors in Mes-13 cells stably transfected with RCAN1.4. Mes-13 cells were stably transfected with an expression vector containing RCAN1.4 construct (RCAN1.4; open bars) or vector alone (GFP; filled bars). From the actively dividing cells in complete medium, mRNAs were prepared and then subjected to analysis for the ECM-related gene expression with specific primers (Table 1). All values are means±SE of at least 5 experiments/group and the values of RCAN1.4-transfected cells are expressed relative to the expression observed in GFP-transfected cells (arbitrarily designated a value of 1). ^*p<0.01, RCAN1.4 vs. GFP group. Col, collagen.