Table 2.

Results of electron microscopical analysis of non-myelinated axons, showing median (interquartile range) values proximal (P) and distal (D) to the repair site, and statistical comparisons between groups (Kruskal–Wallis test)

	Sham	PBS	M6P	P-value
Axon count
P	8163 (6966–9499)	7073 (6068–7367)	6150 (5198–8943)	0.174
D	8982 (6670–12 864)	6611 (4603–10 149)	7108 (4681–14 481)	0.613
Axon density (number per mm2)
P	45 060 (37 720–67 371)	36 880 (29 640–46 787)	32 784	0.112
D	53 823 (40 943–75 416)	35 215 (25 430–53 041)	49 335 (30 277–64 957)	0.264
Non-myelinated : myelinated axon ratio
P	1.72 (1.54–2.31)	1.50 (1.14–1.80)	1.38 (1.10–1.57)	0.093
D	2.12 (1.69–2.38)	1.84 (1.39–2.38)	2.03 (1.46–2.18)	0.651
Remak bundle count
P	955 (849–1105)	1534* (996–2490)	1448† (927–3152)	0.185
D	1165 (584–1243)	3168* (2529–4482)	4241† (2836–6582)	0.007
Remak bundle density
P	4878 (4546–6867)	7768* (5591–12 786)	7231* (5616–13 343)	0.114
D	7025 (3616–7755)	15 557* (13 952–24 261)	28 863* (18 302–29 569)	0.005
Number of axons per Remak bundle
P	8.4 (8.2–10.4)	4.5‡ (3.0–6.5)	4.3‡ (3.0–5.7)	0.018
D	9.9 (8.2–13.4)	2.1‡ (1.8–2.3)	1.7‡ (1.6–2.2)	0.008

Significant differences between proximal and distal values in the same group (Mann–Whitney U-test) were as follows:

^*P = 0.016

^†P = 0.032

^‡P = 0.008.

M6P, mannose-6-phosphate; PBS, phosphate-buffered saline.