Skip to main content
. Author manuscript; available in PMC: 2012 Nov 17.
Published in final edited form as: Neuron. 2011 Nov 17;72(4):559–571. doi: 10.1016/j.neuron.2011.09.032

Figure 2. Rescue of developmental defects of newborn neurons from FEZ1 knockdown by overexpression of wild-type FEZ1.

Figure 2

(A) Sample confocal images of double-labeled newborn neurons in the adult dentate gyrus. Retroviruses co-expressing mCherry and shRNA-FEZ1#1 (F1) and those co-expressing GFP and mouse FEZ1 (mFEZ1) or GFP alone (pCUXIE) were co-injected into the adult dentate gyrus and animals were analyzed at 14 dpi after immunostaining for GFP. Shown are sample confocal projection image of GFP staining (left) and images of GFP staining (green), mCherry (red), DAPI (blue) and merged at lower magnification. Scale bar: 10 μm.

(B and C) Summary of total dendritic length (B) and soma size (C) of newborn neuron in the adult brain expressing shRNA-control (C1), mFEZ1, shRNA-FEZ1#1 (F1), or co-expressing F1 and mFEZ1 (F1 + mFEZ1) or control vector (F1 + pCUXIE). Numbers associated with each bar graph refer to the total number of neurons analyzed from at least two animals under each condition. Values represent mean ± SEM (*: P < 0.01; ANOVA).