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. Author manuscript; available in PMC: 2012 Nov 17.
Published in final edited form as: Neuron. 2011 Nov 17;72(4):559–571. doi: 10.1016/j.neuron.2011.09.032

Figure 3. Functional interaction between FEZ1 and DISC1 in regulating development of newborn neurons in the adult brain.

Figure 3

(A-E) Effect of double knockdown of DISC1 and FEZ1 on development of newborn neurons in the adult dentate gyrus. Shown in (A) are sample confocal projection images of GFP+ newborn neurons expressing shRNA-control (C1), shRNA-FEZ1#1 (F1), a weak shRNA against mouse disc1 (shRNA-DISC1#3; D3), or a strong shRNA against mouse disc1 (shRNA-DISC1#1; D1) at 14 dpi. Shown in (B) are sample confocal images of double-labeled newborn neurons co-expressing shRNA-DISC1#3 (D3)/GFP and shRNA-FEZ1#1 (F1)/mCherry at 14 dpi. Scale bars: 20 μm. Also shown are summary of the total dendritic length (C), Sholl analysis of dendritic complexity (D) and soma size (E) of newborn neuron in the adult brain expressing shRNA-control (C1), shRNA-FEZ1#1 (F1), shRNA-DISC1#3 (D3), or shRNA-DISC1#1 (D1), or co-expressing F1 and D3 (F1 + D3), at 14 dpi. The same group of cells for C1 and F1 as in Figure 1 were plotted for comparison. Values represent mean ± SEM (*: P < 0.01; ANOVA).

(F-H) Effect of expression of a DISC1 blocking peptide on development of newborn neurons in the adult dentate gyrus. Retroviruses co-expressing GFP and a peptide encoding a DISC1 domain that interacts with FEZ1 (a.a. 446-633; Figure S3D) were stereotaxically injected into adult dentate gyrus to infect proliferating progenitors. Shown are summaries of the total dendritic length (F), Sholl analysis (G) and soma size (H) of GFP+ neurons at 14 dpi, similarly as in (C-E).