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. Author manuscript; available in PMC: 2012 Nov 17.
Published in final edited form as: Neuron. 2011 Nov 17;72(4):559–571. doi: 10.1016/j.neuron.2011.09.032

Figure 5. Biochemical interaction among FEZ1, DISC1 and NDEL1.

Figure 5

(A) Association of endogenous DISC1, FEZ1 and NDEL1. Cultured adult mouse neural progenitors were subjected to co-IP analysis using antibodies against DISC1, NDEL1 or FEZ1, respectively. A summary of quantification of co-IP efficacy is also shown. Values represent mean ± SEM (n = 3; P > 0.05; ANOVA).

(B) Lack of interaction between FEZ1 and NDEL1 in adult neural progenitors with DISC1 knockdown. Adult neural progenitors were infected with retroviruses to express shRNA-DISC1 (D1) or shRNA-control (C1). After 48 hours, cell lysates were subjected to Western Blot analysis for expression of DISC1, FEZ1 or NDEL1, or subjected to co-IP analysis using antibodies against FEZ1 or NDEL1, respectively. A summary of quantification of co-IP efficacy is also shown. Values represent mean ± SEM (n = 3; *: P < 0.01; ANOVA).

(C) Independent interaction between DISC1 and FEZ1 and between DISC1 and NDEL1. Adult neural progenitors were infected with retroviruses to express shRNA-NDEL1 (N1), shRNA-FEZ1 (F1), or shRNA-control (C1). After 48 hours, cell lysates were subjected to co-IP analysis using antibodies against DISC1. A summary of quantification of co-IP efficacy is also shown. Values represent mean ± SEM (n = 3; P > 0.05; ANOVA).