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. Author manuscript; available in PMC: 2012 Nov 17.
Published in final edited form as: Cell Host Microbe. 2011 Nov 17;10(5):515–526. doi: 10.1016/j.chom.2011.09.012

Figure 4. PAR-CLIP targets of KSHV miRNAs are downregulated at the mRNA level.

Figure 4

(A) Primer extension analysis of BJAB cell pools stably expressing KSHV miR-K1 (upper) or miR-K4-3p (lower). miR-16 served as a loading control. (B) Western Blot analysis of TPD52 and PPP2CA expression in untransduced BJAB cells and transductants stably expressing the control vector or the indicated miRNA. GAPDH expression served as loading control. (C) Normalized enrichment scores (NES) and FDR q-values for the enrichment of PAR-CLIP targets of the indicated miRNA from BC-1 or BC-3 libraries in the corresponding microarray data. Also shown are data for the enrichment of either 7mer1A or 7mer2-8 seed matches in the array data. (D) Gene set enrichment plots showing the enrichment of PAR-targets of the indicated miRNA from BC-1 or BC-3 libraries in the corresponding rank-ordered gene lists. See also Table S10.