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. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Stem Cell Res. 2011 Aug 16;8(1):26–37. doi: 10.1016/j.scr.2011.08.002

(A) Schematic diagram of inducible strategy in a lentiviral vector. The human Bcl-xL gene was driven by a tetracycline inducible promoter. The constitutive expression of GFP was driven by a human EF-1alpha promoter. (B) Western blot analysis of Bcl-xL in H1-Bcl-xL cells. Different concentrations of doxycycline were tested to induce Bcl-xL expression in the H1-Bcl-xL cells. H1-GFP cells were used as control hESCs. (C) Decrease of apoptotic cells in H1-Bcl-xL cells. HESCs were dissociated into single cells. Activated caspase-3⁺ cells in the presence of doxycycline (500 ng/ml) were determined by FACS every three hours. Data are representative of three independent experiments. * p<0.05 is considered statistically significant.

(A) Schematic diagram of inducible strategy in a lentiviral vector. The human Bcl-xL gene was driven by a tetracycline inducible promoter. The constitutive expression of GFP was driven by a human EF-1alpha promoter. (B) Western blot analysis of Bcl-xL in H1-Bcl-xL cells. Different concentrations of doxycycline were tested to induce Bcl-xL expression in the H1-Bcl-xL cells. H1-GFP cells were used as control hESCs. (C) Decrease of apoptotic cells in H1-Bcl-xL cells. HESCs were dissociated into single cells. Activated caspase-3⁺ cells in the presence of doxycycline (500 ng/ml) were determined by FACS every three hours. Data are representative of three independent experiments. * p<0.05 is considered statistically significant.