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. 2011 Nov 15;25(22):2347–2360. doi: 10.1101/gad.173443.111

Figure 5.

Figure 5.

ARL3, UNC119b, and RP2 are required for ciliary localization of NPHP3. (A) RPE cells stably expressing LAP7-NPHP3 1–200 transfected with siRNAs against the indicated genes and immunostained with pericentrin (PCNT) and acetylated tubulin (AcTub) antibodies. Bar, 3 μm. (B) Quantification of the percentage of cilia containing LAP7-NPHP3 1–200 after transfection with the indicated siRNAs. Experiments were performed in duplicate and are presented as average plus standard deviation. (C) RPE cells stably expressing LAP7-NPHP3 1–200 were transfected with tdTomato-tagged wild-type or Mut-4 UNC119b, then transfected with siRNAs targeting the UNC119b 3′ UTR. The cells were then immunostained for pericentrin and acetylated tubulin and imaged. The arrow indicates the basal body, and the arrowhead indicates the cilium. Bar, 3 μm. (D) Quantification of the percentage of cilia containing LAP7-NPHP3 1–200 after transfection with the indicated plasmids and siRNAs. Experiments were performed in duplicate and are presented as average plus standard deviation.