Figure 6.

CLK binding can be tissue-specific. CLK ChIP–chips were performed on either wild-type (CLK-V5) or GMR-hid (CLK-V5, GMR-hid) flies at ZT14. In GMR-hid flies, the majority of the eye tissue is ablated. We compare CLK ChIP signal in wild-type (red) and GMR-hid (blue) flies on six different genes. CLK binding is undetectable on gol (A) and mnt (B) when eyes are ablated, suggesting that gol and mnt are CLK direct targets primarily in eye tissue. CLK binding is unaffected on lim1 (C) and crp (D) in GMR-hid, suggesting that these genes are CLK targets in non-eye tissue. Interestingly, on some genes with multiple CLK promoter peaks, the two peaks of CLK binding are differentially affected in GMR-hid. One of the peaks of CLK binding on both pdp1 (E) and lk6 (F) is greatly diminished in GMR-hid, while another peak is much less affected.