Figure 3.

The yeast PUA domain and DC mutations. (A) Domain organization of yeast Cbf5. The NTE, catalytic domain (CAT), PUA domain, CTE, and KKE/D repeat are labeled. (B) Structural alignment of the PUA domain of yeast and Pf Cbf5. The NTE of yeast Cbf5, colored in magenta, forms an extra structural layer on the PUA domain and is divided into loops L1, L2, and L3. The ACA motif of H/ACA RNA bound to the Pf PUA domain is shown as sticks. Dots denote disordered residues. (C) DC mutations surrounding the NTE. DC mutation sites mapped to the yeast Cbf5 structure are marked by Cα spheres (orange) and yeast residue names, followed by the amino acids found in DC.