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The CTE allows Gar1 to bind Cbf5 tighter than Naf1. Copurified Cbf5C–Nop10 and Cbf5C–Nop10–Gar1C and Cbf5C–Nop10–Gar1CΔ120 complexes (200 pmol) were incubated with 0, 50, 200, 800, and 3200 pmol of Naf1C and pulled down by Ni beads. Only Cbf5C contains a His6 tag among these proteins. Lanes 1 and 2 show retention of 200 and 3200 pmol of Naf1C. Lanes 3, 9, and 15 are 60 pmol of Cbf5C complexes prior to pull down.
