Figure 7.

Interaction between Nhp2 and Nop10 analyzed by chemical cross-linking and mass spectroscopy. (A) A representative fragmentation spectrum of a BS3 cross-linked peptide pair consisting of FSPDDKYSR (α peptide) from Nop10 and EVVKALR (β peptide) from Nhp2. The observed C-terminal fragments (y ions) resulting from a single event of peptide bond cleavage are highlighted in green if the cleavage occurs on the α peptide or blue if it occurs on the β peptide. The corresponding N-terminal fragments are called b ions, from which a further loss of carbonyl results in a ions. As is typical of collision-induced dissociation, y ions are the dominant species. Only b2⁺, a1⁺, and a2⁺ from the breakage of the α peptide are observed. (B) Three cross-linked lysine pairs between Nop10 and Nhp2 are shown in the model of yeast Nhp2 protein complex.