Fig. 3.

PCNA expression and distribution in mouse PNET cells and in mouse fibroblasts. Western blot analysis from total lysates shows similar expression levels of PCNA in all PNET cells (A, B). In the T-Ag-negative PNET cells, PCNA accumulates in the cytoplasm, whereas in the T-Ag-positive PNET cells PCNA is detected in both the nuclear and cytoplasmic fractions (C, D). Efficient cytoplasmic and nuclear fractionation was confirmed by Western blotting analysis using anti-α-tubulin antibody for the cytoplasmic fraction and antilamin A/C antibody for the nuclear fraction.