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. Author manuscript; available in PMC: 2011 Nov 23.
Published in final edited form as: J Cell Biochem. 2010 May;110(1):182–190. doi: 10.1002/jcb.22525

Fig. 4.

Fig. 4

pRb family expression in human medulloblastoma cells (DAOY, D283) and human fibroblast cells (WS-1). The relative protein levels of pRb/p105, p107, and pRb2/p130 were detected by Western blotting using total (A) or nuclear or cytoplamic (B) extract. The expression of α-tubulin protein was assessed to normalize protein loading (A). Efficient cytoplasmic and nuclear fractionation was confirmed by Western blotting analysis using anti-α-tubulin antibody for the cytoplasmic fraction and antilamin A/C antibody for the nuclear fraction (B).