Changes in glycolysis and sucrose metabolism during citrus fruit development. (a) Glycolysis and sucrose biosynthesis pathway. Enzymes that were found to be differentially expressed are numbered with a black background. Enzymes that remain unchanged are numbered with a white background. (1) Sucrose synthase. (2) Invertase. (3) UDP-Glu-pyrophosphorylase. (4) Hexokinase. (5) Fructokinase. (6) Glucose-6-P-isomerase. (7) Phosphoglucomutase. (8) Sucrose-phosphate-synthase. (9) Sucrose-phosphatase. (10) ATP-dependent phosphofructokinase. (11) PPi-dependent phosphofructokinase. (12) Fructose-bisphosphate aldolase. (13) Triose-phosphate-isomerase. (14) Glyceraldehyde-3-phosphate dehydrogenase. (15) Phosphoglycerate kinase. (16) Phosphoglycerate mutase. (17) Enolase. (18) Pyruvate kinase. (19) PEP carboxylase. (20) Malate dehydrogenase. (b) Protein expression changes of the above proteins during the transition from early stage II to stage II and from stage II to stage III. The figure shows enzyme changes as a function of development. The x-axis represents the three stages sampled: 35, early stage II; 55, stage II; 80, stage III. The y-axis represents the calculated fold change ratio where stage II was assigned the value 1 and the values in early stage II and stage III were calculated according to the fold change found by dMS. An average of the different isoforms was calculated in cases where different protein isoforms were found (see also Table 1). (c) Changes in patterns of gene expression of invertases (βFruct1, βFruct2, CitCNV1), sucrose synthase (CitSUSA), glucose-6-P-isomerase (G6PI), PPi-dependent phosphofructokinase 1 and 2 (PFP1 and PFP2), ATP-dependent phosphofructokinase (PFK1), sucrose-phosphate-synthase (SPS), and sucrose-phosphate phosphatase (SPP) during fruit development. The x-axis represents the three developmental stages as described in (b). (d) Sucrose-phosphate-synthase (SPS) and Sucrose-phosphate phosphatase (SPP) activities in juice sacs extract during fruit development.