Fig. 4.

BoCCD1 gene location in seed preparations (A–D) and in the dissected aril layer (E–H) from the immature seeds of B. orellana. Seed and dissected fresh aril preparations are shown in A and E. In situ RT-PCR tissue preparations are shown in B–D and F–H, with the following treatments: (B) and (F) BoCCD1 amplification; (C) and (G) actin amplification; (D) and (H) digestion with RNase prior to BoCCD1 amplification. Arrowheads point to RT-PCR-positive cells. A, B, and E are unstained tissue sections Tissue abbreviations: ar, aril; oc, oily cells; en, endosperm. Scale bars=400 μm.