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Comparison of fluorescence techniques in root cells. Comparison of TIRFM, VAEM, epifluorescence, and white light transmission of EGFP in root cells. The angle of incident light was measured to determine the technique used and hence compare them. Scale bars=10 μm. (a) Endoplasmic reticulum (ER) marker (mGFP5–ER). (b) Microtubule marker (MAP4–GFP).
